Composite
Part:BBa_K3431049:Design
Designed by: Huan-Jui Chang, Cheng-Ruei Yang, Hung-Yu Chen Group: iGEM20_CSMU_Taiwan (2020-10-17)
Banana_ToeholdSwitch-Regulated amilCP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We included Strong T7 Promoter and Double T7 Terminator to ensure optimal performance of this expression device, and further compared the result with previous construct of amilCP (Blue Chromoprotein) (BBa_K1087003)
Source
We obtain the sequence of amilCP (Blue Chromoprotein) from BBa_K592009, and synthesized the construct through IDT.